Streaking for Single Colonies: The Streak Plate Challenge
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Quadrant Streaking It is the most commonly used and the most preferred method where four equal-sized sections of the agar plate are streaked. It is also referred to as the “four-quadrant streak” or “four sectors” or “four-way streak” method. In this method, each plate is divided into four equal sectors and each adjacent sector is streaked sequentially. The sector which is streaked first is called the first sector or the first quadrant, and it has the highest concentration of inoculum. Gradually
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