Gene syntax also affects the behavior of genetic circuits, and contributes to unpredictable outcomes in genetic networks.

when one plasmid contains a library), we prepare electrocompetent cells containing the constant partner. Figure 2: (a) Schematic plate layout for screening interactions with the GFP reassembly screen.

Distribution of Ranvier nodes along myelinated axons is a critical determinant of conduction velocity, significantly influencing spike arrival timing and hence neural circuit function. The patterns of ...

To test this approach, we inserted a green fluorescent protein (GFP) nanobody (GFPnb) into the VP1 protein of AAV-DJ inside the capsid and replaced the AAV payload plasmid with a GFP expression ...

were downloaded from the National Center for Biotechnology Information and ligated into a pCDH-CMV-MCS-EF1-GFP-puro vector by Changsha Youbao Biotechnology Co., Ltd. (Changsha, China). The packaging ...

The data convincingly show that the transcription factor Sp1 regulates ephrin-mediated axon guidance in the spinal cord. Although the authors show that Sp1 and its co-activators p300 and CBP are ...

The role of centrosomal tubulin glutamylation has remained elusive for decades. Here, a new tool enables precise ...

How chromatin openness controls behavioral changes remains unclear. This work implicates alternative promoter selection, ...

A team recently detailed an auto-expression medium to maximize protein production that uses galactose rather than lactose to induce auto-expression.

Nanotechnology offers a next-gen solution to plant genome editing challenges by enabling precise, efficient delivery of CRISPR, RNA, and DNA tools using nanoparticles like AuNPs, CNTs, and lipid ...

Deep below the Earth's surface, rock and mineral formations lay hidden with a secret brilliance. Under a black light, the chemicals fossilized within shine in brilliant hues of pink, blue and green.