which increases specificity through a combination of chemical modifications and better sequence-selection strategies to promote guide-strand uptake at low concentrations of siRNA. Other groups are ...

A significant cause of these off-target effects is a seven-nucleotide region known as the seed region, located within the guide strand of the siRNA, which is critical for target recognition.

The discovery of siRNA and its role in RNAi was a groundbreaking finding in the field of molecular biology. In 1998, Andrew Fire and Craig Mello published a seminal paper describing the phenomenon of ...